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Results In cars compliant with the new regulations, gasoline (E1 displayed the highest PM concentrations and toxicological responses, while the higher ethanol blend (E85) resulted in slightly lower exhaust gas PM concentrations and notably lower toxicological responses in comparison. Engines powered by modern diesel and compressed natural gas (CNG) yielded the lowest PM concentrations and toxicological responses. Conclusions The present study shows that toxicity of the exhaust gas PM varies depending on the fuels used. Additionally, c
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