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The positive effects of nucleotide (NU) supplementation in milk replacer have been elucidated in infants and in dairy calves; however, NU addition to whole milk has not been evaluated previously. This study aimed to assess NU supplementation in the whole milk on calf growth and health. Thirty Holstein calves (body weight 39.1 ± 1.0 kg; 3 d after birth) were randomly assigned to the following treatments whole milk without any supplementation (NU, whole milk + 0.5 g/d added a NU-containing supplement to whole milk (NUCS0.5), and whole