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RNA-seq analysis of mature leaves indicated that overexpression of Pu-miR172d decreased the expression of many genes related to photosynthesis. Our findings show that the Pu-miR172d/PuGTL1/PuSDD1 module plays an important role in stomatal differentiation, and hence it is a potential target for engineering improved drought tolerance in poplar.Instantaneous electrical responses in plants have been widely studied, but the mechanism of spontaneous, periodic electric potential alternations in the xylem of tree trunks remains controversial. Th