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Artisanal and small-scale gold mining (ASGM) is the largest global source of anthropogenic mercury emissions. However, little is known about how effectively mercury released from ASGM is converted into the bioavailable form of methylmercury in ASGM-altered landscapes. Through examination of ASGM-impacted river basins in Peru, we show that lake area in heavily mined watersheds has increased by 670% between 1985 and 2018 and that lakes in this area convert mercury into methylmercury at net rates five to seven times greater than rivers. The