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Seasonal nitrogen (N) cycling in Populus, involves Bark Storage Proteins (BSP) that accumulate in bark phloem parenchyma in the fall and decline when shoot growth resumes in the spring. Little is known about the contribution of BSP to growth or the signals regulating N remobilization from BSP. Knockdown of BSP accumulation via RNAi and N-sink manipulations were used to understand how BSP storage influences shoot growth. Reduced accumulation of BSP delayed bud break and reduced shoot growth following dormancy. Further, 13N tracer studies
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