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A pot study was performed to evaluate the influence of O3 stress with different biochar treatments on a wheat cultivar (HD 2967). Plants were subjected to ambient and elevated (ambient+20 ppb) O3 along with three doses of biochar (0%, 2.5%, and 5%). Elevated ozone alone reduced most of the growth parameters, negatively affecting the test cultivar's physiology. Although enzymatic antioxidants were up-regulated by elevated O3, damage to the membrane integrity was evident by higher MDA content in the wheat leaves. Besides, the uptake of n