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The different chemical spaces of metabolites and lipids require different specificities, hence different acquisition and data processing approaches must be considered for their analysis. Conclusions Although the hardware and acquisition modes are well defined for SWATH-MS, a major challenge for routine use remains the lack of appropriate software tools capable of handling large datasets and large numbers of analytes.Leishmaniasis is a neglected tropical disease with no effective vaccines to date. Globally, it affects around 14 million peo