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At exactly the same time, there were unique potential mutant epitopes (binding affinity not as much as 50 nM) in case there is HLA-A*3201 allele. Interestingly, mutations caused multidirectional impact on the binding affinities of the viral peptides and HLA-DRB1*0301. Particularly, Spike protein mutations when you look at the BA.1 variant caused more than 100-fold decrease in PINLVRDLPQGFSAL binding affinity, 10-fold decline in affinity in the case of BA.2, BA.4, and BA.5 variants, and 30% increase in affi