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The MOV, MFD and MFN remained unchanged after surgery in four groups, and the menstrual cycle after surgery at 1 month in group D delayed (P0.05). The use of CO2 for pneumoperitoneum affects the levels of E2, LH, and FSH during the first menstruation after laparoscopic surgery in a pressure-dependent manner and the impact vanishes by the third menstruation. Chinese Clinical Trial Registry ChiCTR2100046221. Chinese Clinical Trial Registry ChiCTR2100046221. A larger volume of local anesthetic provides a wider range of blocked sensory but