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Therefore, this molecular framework provides a new way of thinking about detecting multiple target substances.The demand for gluten-free banana flour has led manufactures to enforce strict measures for quality control. A need has arisen for the development of more sensitive and reliable methods to test the quality of green banana flour (GBF). The objective of this study was to develop rapid visible to near-infrared (Vis-NIR) based spectroscopic models to detect gluten concentration, as a biomarker to detect wheat flour adulteration in green banana f