https://www.selleckchem.com/pr....oducts/Temsirolimus.
Bio-catalysis by enzymes on solid surfaces has been implemented in several practical applications. However, the current methods for efficient enzyme immobilization with retained activity need further development. Herein, a simple, rapid, and economical, bio-affinity-based approach was developed for the direct immobilization with high activity recovery of recombinant Bacillus subtilis catalase (CAT) from the crude lysates. Silaffin-3-derived pentalysine cluster (Sil3K) and its mutant variant (penta-arginine peptide; Sil3R) were used
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