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After lipopolysaccharide (LPS) treatment, cytokine levels had been calculated using ELISAs, NF-κB transcription element activity had been detected making use of EMSA and necessary protein phrase levels had been analyzed utilizing Western blot assays subsequently in EpCs and MPs. Co-cultured EpCs/MPs were discovered to exude increased levels of interleukin (IL)-6, IL-1β, IL-8 and tumor necrosis element (TNF)-α following LPS exposure for 6, 12, 24 and 48 h compared wi
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