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STUDY DESIGN Cross-sectional study. OBJECTIVE To determine the prevalence and potential risk factors of vitamin D deficiency and insufficiency among Malaysian children with spina bifida. SETTING Four Malaysian tertiary hospitals. METHODS Children with spina bifida were assessed for potential demographic, disease severity and lifestyle risk factors for vitamin D deficiency and insufficiency. Blood for 25-hydroxy vitamin D (25(OH)D) was taken. Vitamin D deficiency was defined as 25(OH)D levels ≤ 37.5 nmol/L and insufficiency as 37.6-50 n