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To investigate the concordance between aqueous and vitreous tap culture results among different types of bacterial endophthalmitis. This retrospective cohort analysis included all cases diagnosed with endophthalmitis at Moorfields Eye Hospital between January 2008 and March 2020. Aqueous and vitreous samples obtained simultaneously at presentation. Samples were evaluated for sensitivity, specificity, negative and positive predictive values. A total of 217 patients (217 eyes) were included in the study. Postsurgical endophthalmitis was t