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Biofilm quantification showed high in vitro biofilm growth for sp., and in almost all culture media excluding Brucella broth. Contrary, only New York City III broth was able to promote biofilm formation for , and . Our data demonstrate that New York City III broth relative to the other tested media is the most conducive for future studies addressing polymicrobial biofilms development as this culture medium allowed the formation of significant levels of single-species biofilms. Our data demonstrate that New York City III broth relative to