https://www.selleckchem.com/pr....oducts/rhapontigenin
Dendritic cells (DCs) regulate processes ranging from antitumor and antiviral immunity to host-microbe communication at mucosal surfaces. It remains difficult, however, to genetically manipulate human DCs, limiting our ability to probe how DCs elicit specific immune responses. Here, we develop a CRISPR-Cas9 genome editing method for human monocyte-derived DCs (moDCs) that mediates knockouts with a median efficiency of 94% across 300 genes. Using this method, we perform genetic screens in moDCs, identifying mechanisms by wh
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