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Unlike traditional methods, the shape and scope of the urban center identified using the proposed method are not restricted by administrative boundaries. Moreover, the level differences of urban centers and the overall urban center development level within a polycentric city, can be expressed by quantitative indicators, which helps in comparing the differences between urban centers.The first and limiting step of nitrification is catalyzed by ammonia-oxidizing archaea (AOA) and bacteria (AO. Recently, complete ammonia oxidizers (comamm