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We determined the prevalence of weight to antibiotics in Escherichia coli isolates collected from wildlife in 2013 and 2014 and the genetic foundation for opposition to third-generation cephalosporin in Guadeloupe. We recovered 52 antibiotic-resistant (AR) E. coli strains from 48 associated with 884 (5.4%) wild animals tested (46 iguanas, 181 birds, 289 anoles, and 368 rats at 163 sampling internet sites). Rats had higher rates of carriage (n = 38, 10.3%) than reptiles and birds (2.4% and 1.1percent, respecti