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1 mm vertebrae 2 (P = 0.061), 0.3 mm phantom 1 (P = 0.209) and 0.3 mm vertebrae 2 (P = 0.097). CONCLUSION This study demonstrated that changing the SRI influences the spatial replication accuracy of 3D-printed models constructed by FDM. Matching the SRI to the primary spatial resolution limiting factor of acquisition slice width or printer capabilities optimises replication accuracy. © 2020 The Authors. Journal of Medical Radiation Sciences published by John Wiley Sons Australia, Ltd on behalf of Australian Society of Medical Imag