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nderpowered and creating a high possibility of type II error. Copyright © 2020 Johnson, Holcombe, Shearer, Watson, Gandy, Southwood, Lynch, Schroeder, Fogle and Sordillo.High impact, mosquito-borne flaviviruses such as West Nile virus (WNV), Usutu virus (USUV), Japanese encephalitis virus (JEV), Tembusu virus (TMUV), and Bagaza/Israel turkey meningoencephalomyelitis virus (BAGV/ITV) are emerging in different areas of the world. These viruses belong to the Japanese encephalitis (JE) serocomplex (JEV, WNV, and USUV) and the Ntay