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Geodetic observations and large-scale laboratory experiments show that seismic instability is preceded by slow slip within a finite nucleation zone. In laboratory experiments rupture nucleation is studied mostly using bare (rock) interfaces, whereas upper crustal faults are typically filled with gouge. To investigate effects of gouge on rupture nucleation, we performed a biaxial shearing experiment on a 350 mm long saw-cut fault filled with gypsum gouge, at room temperature and a minimum horizontal stress σ2 = 0.3-5 MPa. The gouge layer