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The eukaryotic expression plasmid pEGFP-PoIFN-λ1 was constructed through the pig IFN-λ1 gene fragment and transfected into CHO-K1 cells. Expression was recognized by fluorescence microscopy and Western blotting. The impact on the proliferation of PRRSV had been assessed. The outcome regarding the research indicated that the recombinant plasmid pEGFP-PoIFN-λ1 had been built correctly. After transfection, green fluorescent signal was detected in CHO-K1 cells by fluorescence
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