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Bacillus, Pseudomonas, Nevskia, and Lactococcus were the keystone genera in this winter wheat-summer maize rotation system on the Loess Plateau. Strong correlations between changes in soil properties and soil bacterial diversity and abundance were identified. In summary, we suggest that the WRNF treatment, as a no-mulching film and no-deep tillage sowing method, would be the most suitable sowing technique in the winter wheat-summer maize rotation on Loess soil. Copyright © 2020 Huang, Han, Yang, Chen and Rengel.The natural product pn