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9%, respectively compared to the blank reactors. The performance and behavior of microbial fuel cells (MFCs) are influenced by among others the external load (Rext). In this study, the anode-surface biofilm formation in MFCs operated under different Rext selection/tracking-strategies was assessed. MFCs were characterized by electrochemical (voltage/current generation, polarization tests, EIS), molecular biological (microbial consortium analysis) and bioinformatics (principal component analysis) tools. The results indicated that the MFC