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The rapid category and sensitive and painful recognition of germs tend to be essential steps of bacterial community study therefore the remedy for illness. Herein, we created optical property-superior and heavy metal-free ZnCuInSe quantum dots (QDs) for achieving fast discrimination of Gram-positive/Gram-negative germs by the naked eye; driven by the structural variations of bacteria, ZnCuInSe QDs are effective in binding to Gram-positive germs, specifically Staphyl