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OBJECTIVES Colibacillosis caused by avian pathogenic E. coli (APEC) is considered as a major hindrance in poultry farming worldwide. This study aimed to characterize the genetic content and the relatedness between multidrug-resistant E. coli isolates from broiler chickens died due to colibacillosis in three farms from Tunisia. METHODS One hundred samples were collected from chickens' fresh carcasses from 3 poultry farms in Tunisia. E. coli isolation and identification were performed. Then, antimicrobial susceptibility regarding antibio
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