https://www.selleckchem.com/pr....oducts/gsk3368715.ht
The optimum conditions for maximum production of the recombinant ficin enzyme in E. coli were as follows; a cell density of 1.25, post-induction time 7 h, 10% (w/v) lactose concentration, and shaking at 115 rpm at 24 °C. The concentration of purified product was reported to be 0.27 mg/ml. The optimization procedures increased the amounts of ficin production by approximately 3 folds (0.67 mg/ml) compared with the expiration level (in the absence of optimization). Also, our findings showed that the recombinant ficin was able to hydroly
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