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This protocol defines exactly how SDS-PAGE and silver staining could be used to figure out the purity of an rAAV planning. In inclusion, using an extremely purified rAAV preparation whose particle titer is famous, this assay could be used to derive a semiquantitative estimate associated with particle concentration of a test vector.Negative staining is a straightforward and quick way of learning the morphology and ultrastructure of tiny particulate specimens (e .g., viruses, bacteria, cellular fragments, a
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