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36 nM. In addition, the developed aptamer sensing method also shows a good selectivity for kanamycin against other interfering antibiotics, and can realize the monitoring of kanamycin added in milk samples, highlighting its potential for sensitive monitoring of trace amount of kanamycin for food safety applications.In spatial comprehensive three-dimensional chromatography (3D-LC) components are separated within a three-dimensional separation space that can lead to unprecedented resolving power, in terms of peak capacity and peak-productio