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48). However, significant decrease of baseline mean VAS pain score of the participants in both groups was found during access cavity preparation or initial file insertion (P0.05). Mixed Li-KT solution did not increase the success rate of IANB injection significantly. Mixed Li-KT solution did not increase the success rate of IANB injection significantly. The present clinical study investigated the microbiota of teeth with persistent secondary endodontic infection in the different phases of root canal retreatment. Twenty filled single-