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The NHE led to higher muscle activation than the other exercises ( 0.001). When exercise type and muscle interaction were examined, SM activation was lower than BF ( = 0.04) and ST ( = 0.001) during NHE ( 0.05). The highest level of muscular activation was recorded during the NHE in both male and female participants. The NHE may be the most effective exercise for the hamstring muscles as it leads to greater muscle activation. SLDL, USLDL, and BLC exercises may be preferred at the beginning of strength training programs since th