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25ng/mL and good-quality embryos and between Hcy concentration (FF) 4.9nmol/mL and clinical pregnancy. OCM in FF may affect fertilization, embryo quality, and clinical pregnancy. OCM in FF may affect fertilization, embryo quality, and clinical pregnancy. Chromosomal abnormalities are a major cause of spontaneous abortion, and conventional G-banded karyotyping (G-banding) is mainly utilized for chromosomal analysis. Recently, next-generation sequencing (NGS) has been introduced for chromosomal analysis. Here, we aimed to investigate