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Developing the new crop varieties with high productivity under low nitrogen (N) input is an important access to facilitate modern agricultural sustainability. In the present study, 20 broomcorn millet (Panicum miliaceum L.) varieties were characterized for their morphological and nutrient parameters to different low N levels in seedling. The results showed that 0.25 mM NH4NO3 was the standard concentration for the evaluation and identification of low-N tolerance. Through pearson's correlation analysis, principal component analysis, and