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The spectral separation of differently colored food dyes was achieved by the fully addressable driver controlled RGBW LEDs. The software was newly written in R to speed-up the processes, supported by the new Raspberry Pi 4B computer with 4 GB RAM. The analysis of Stevia leaves for steviol glycosides yielded results comparable to the status quo. Different water samples were analyzed for bioactive compounds. Thereby, compounds of general cytotoxicity were effect-directed detected by bioluminescent A. fischeri bacteria. It allowed the bioana