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For all 3 metals, we found that concentrations hazardous for 5% of the species (HC5 s) based on MSA relationships are systematically higher than SSD-based HC5 values. Our proposed framework can be useful to derive abundance-based ecological protective criteria for chemical exposure, and creates the opportunity to assess abundance impacts of chemical exposure in the context of various other anthropogenic stressors. Environ Toxicol Chem 2020;392304-2313. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodi