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INTRODUCTION To develop a new method to quantify the density of nerves, vessels and the neurovascular contacts, we studied skin biopsies in diabetes and control subjects. METHODS Skin biopsies with dual immunofluorescent staining were used to visualize nerves and blood vessels. The density of nerves, vessels, and their neurovascular contacts were quantified with unbiased stereology. Results were compared to examination findings, validated questionnaires and autonomic function. RESULTS In tissue from 19 controls and 20 patients with di