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The association of the TRS with AD diagnosis and entorhinal cortical thickness was also replicated in AddNeuroMed. Among functional genes identified to calculate the TRS, and were significantly upregulated, and was significantly downregulated in patients with AD compared with CN, all of which were identified in the ADNI and replicated in AddNeuroMed. The blood-based TRS is significantly associated with AD diagnosis and neuroimaging biomarkers. In blood, and were known to be associated with uptake of β-amyloid and herpes simplex virus 1 i