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Quick and efficient assessment techniques have to accelerate the development of cultivars for razor-sharp eyespot weight in grain breeding. Here, a two-step colonized grain kernels (TSCWK) method for the inoculation and classification of sharp eyespot weight in seedlings ended up being established in a greenhouse. After preliminary confirmation of the reliability regarding the method in 2 replicates, 196 grain cultivars were evaluated for razor-sharp eyespo