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03). We propose that mechanical force drives fluid secretion through the tight junction complex via a "one-way check valve" that can be regulated. This pathway of filtration secretion complements chloride-coupled fluid secretion during high-volume fluid flow. The role of filtration secretion in the genesis of diarrhea in intact animals needs further study. Our findings may explain a potential linkage between intestinal motility and intestinal fluid dynamics.We synthesized and subsequently rationalized the formation of a series of 3D hi