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Besides contributing to background decrease, the CoOOH nanoflakes strongly adsorbed nucleic acids and isolated them from a complex test matrix, thus permitting effective detection of the target miRNA in the serum. We anticipate that facile but sensitive template-blocking EXPAR could be an invaluable device to support the development and validation of miRNA markers in biospecimens. Graphical abstract.A collection of untargeted techniques happens to be requested the characte