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Solids loading is a key factor in aqueous or gaseous ammonia production from urea. Methane production from urea-pretreated corn stover, as well as the physicochemical characteristics and delignification kinetics of the corn stover, were investigated with four solids loading values (10%-70%) and five ratios of urea to corn stover (1100-71 at 35 °C for 6 weeks. A 120 ratio of urea to corn stover was optimal for achieving high lignin removal with ≤50% solids loading, and 710 was optimal with 70% solids loading. Under the two optimal cond