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AIM To evaluate the antimicrobial, toxicity, and cleaning effectiveness of ethylenediaminetetraacetic acid (EDTA) and Maleic acid (MA) alone and combined with cetrimide (CTR). METHODOLOGY Cytotoxic and genotoxic effects were assessed on Chinese hamster cells V79 using the MTT, clonogenic, and micronucleus assays, respectively. The bacterial inhibitory and bactericidal concentrations (MIC and MBC, respectively) were determined on a strain of E. faecalis. Antimicrobial tests were performed on a biofilm model after treatment with the chela