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We discovered a higher prevalence of intra-anal HSIL in females formerly diagnosed with vulvar and perianal HSIL. Given the clear link between HSIL and SCCA, testing for intra-anal lesions in females with HPV-related genital pathology appears warranted. Future studies should concentrate on the effectation of HSIL treatment regarding the avoidance of anal cancer.We found a high prevalence of intra-anal HSIL in women previously diagnosed with vulvar and perianal HSIL. Given the obvious website link be