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The most important variables in species segregation were a deeper olfactory bulb in G. lessae and a greater distance between the bulbs in G. marmorata. Contrary to the body anatomy, the brain anatomy reveals that Gymnura has a simpler and more primitive brain than most derived myliobatoids. Our results are consistent with the evidence from phylogenies developed with molecular data, where gymnurids are a basal group within myliobatoids. Ecological studies have suggested that Cannabis legalization might have led to a decrease in opioid ov