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We demonstrated that old mice (24 months) have delayed fracture recovery with significantly less bone tissue and more cartilage compared to young mice (3 months). The quantity of infiltrating macrophages in to the fracture callus was comparable in old and young mice. Nevertheless, RNA-seq analysis shown distinct differences in the transcriptomes of macrophages based on the break callus of old and younger mice, with an up-regulation of M1/pro-inflammatory genes in macrophages f
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