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Compared to rabbit testes, in which there was the least number of senescent cells in seminiferous tubules, there was a greater abundance of senescence markers in both nutria and chinchilla testes (P less then 0.05; P less then 0.001, respectively). Furthermore, there were small abundances of caspase 3 and LC3 in the testes of all species. In chinchilla testes, there was a lesser concentration of cholesterol (P less then 0.001) and testosterone compared with the other species. Cellular senescence in testes, therefore, can be assessed by