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In particular, we demonstrate that T. alpestre has i) a higher level of expression for stv and other heat-shock proteins in chill shock tests, and ii) enzymatic enhancement of Hex-T1, a rate-limiting regulatory enzyme that controls the entry of glucose into the glycolytic pathway. Together, our analyses highlight the adaptive molecular changes that support colonisation of high-altitude environments. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.OBJECTIVE Wh