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Additionally, our findings provide theoretical support for using superfine grinding in industrial food applications. © 2020 The Authors. Food Science Nutrition published by Wiley Periodicals, Inc.The chemical composition and anti-inflammatory activity of gypenosides isolated from tetraploid Gynostemma pentaphyllum (GP) leaves were investigated. The gypenosides accounted for 7.43 mg/g of the tested GP sample, which were composed of four major saponins including isomers of gypenoside 1 and 2 (C47H76O18), 3 (C47H76O17), and 4 (C46H7