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In-vivo passage of viruses is an alternative to in vitro culture to produce inocula to better study the pathogenesis of infection and more rigorously and relevantly assess vaccine efficacy.Lymphatic imaging and interventions are gaining wider acceptance as the treatment of various lymphatic diseases. Meanwhile, the liver lymphatic system remains relatively unknown despite its physiological importance. Liver lymph has been at the center of the lymphatic research since the 19th century; however, the acquired knowledge has not been used i