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© 2020 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.AIMS The objective of the study was to determine levels of Escherichia coli O157H7 colonization in the gastrointestinal tract (GIT) of naturally-shedding cattle shedding the pathogen at low- or super-shedder levels. METHODS AND RESULTS Over two years, feedlot cattle were sampled multiple times for faecal shedding of E. coli O157H7. Just prior to harvest (1 to 2 days), animals that were super-shedders (≥104 CFU g-1 of faeces) were specifically identified, and based on the longer term